张蕾，理学博士，博士生导师，现任中国科学院福建物质结构研究所研究员，2022年入选中国科学院特聘骨干人才。在分子生物学、基因组学、蛋白质功能、生物医用材料等研究领域具有丰富的科研经历和研究基础。1996-2007年于华中农业大学农业微生物国家重点实验室完成本科、硕博研究生学历；2007-2010年在武汉大学生物医用高分子材料教育部重点实验室从事博士后研究，2010年获得武汉大学优秀博士后奖；随后在厦门大学固体表面物理化学国家重点实验室从事科研工作；2012年至今，在中国科学院福建物质结构研究所从事离子通道与肿瘤相关蛋白的结构生物学研究和生物分子靶向纳米运载体的研发，主持了两项国家自然科学基金项目和两项福建省科技项目。2017年至2018年访问学者，从事逆转肿瘤多药耐药性（MDR）的机制及靶向MDR肿瘤纳米药物研究。主要成果包括：通过小分子抑制剂的胞内靶向封闭及CRISPR/Cas9 基因敲除证明了磷酸肌醇三激酶 PI3K 110α/110β为逆转肿瘤多药耐药性的潜在靶点、PI3K-CDK6为逆转P-糖蛋白介导的肿瘤多药耐药性的信号通路轴；在此基础上设计了一种具有肿瘤靶向性的多功能纳米体系PBDF，该体系以金核枝接PLGA-SH包裹的阿霉素（DOX）和BAY-1082439颗粒，利用叶酸介导靶向肿瘤作用，有望克服P-糖蛋白介导的肿瘤多药耐药性；构建了pH敏感的“PMMMA/PLGA/抗原”口服疫苗体系，该体系在酸性的胃及多种酶存在的小肠内提供对抗原的保护作用，大肠中能缓释抗原至免疫相关的细胞，实现抗原呈递和诱导抗体形成；基于肌醇等生物可利用或生物可降解分子构建了系列高效进入动物细胞、靶向肿瘤细胞、或促进基因转染及表达的系列高效低毒副作用的药物传递体系；构建了胞内抗体的展示与筛选系统；证明了苏云金芽胞杆菌CTC菌株的晶体蛋白与表面S-层蛋白同为一种蛋白，为芽胞杆菌分类提出新的依据。在Molecular Cancer、Acta Pharmaceutica Sinica B、ACS AMI、Biomaterials、Journal of Materials Chemistry B、Drug Discovery Today、International Journal of Nanomedicine、Colloid surface B、Applied Microbiology and Biotechnology、Journal of Applied Microbiology 等国内外权威刊物上以第一作者和通讯作者发表了多篇学术论文。获得第十五届福建省自然科学优秀学术论文一等奖和三项国家发明专利。

主要研究方向：生化与分子生物学、生物医用材料、微生物学、药学等学科及其交叉学科，如多靶向抗肿瘤药物体系、新型口服疫苗、基于生物信息学的网络调控等。欢迎有志于科研的同学报考！

邮箱地址: zhangl@fjirsm.ac.cn

办公电话：0591-63001308

个人主页：https://www.researchgate.net/profile/Lei_Zhang124

代表性期刊论文：

1) Lei Zhang*, Yidong Li, Chaohua Hu, Yangmin Chen, Zhuo Chen, Zhe-Sheng Chen, Jian-Ye Zhang*, Shuo Fang*. CDK6-PI3K signaling axis is an efficient target for attenuating ABCB1/P-gp mediated m ulti-drug resistance (MDR) in cancer cells. Mol. Cancer. 2022, 21:103.

2) Lei Zhang, Biwei Ye, Zhuo Chen*, Zhe-Sheng Chen*. Progress in the studies on the molecular mechanisms associated with multidrug resistance in cancers. Acta Pharm. Sin. B. 2023, 13(3): 982-997.

3) Ruikun Lin, Lei Zhang*, Biwei Ye, Yanan Wang, Yi-Dong Li, Jason Hsu, Wenzhen Liu, Ping Hu, Jincan Chen, Zhe-Sheng Chen, Zhuo Chen*. A multi-functional nano-system combining PI3K-110α/β inhibitor overcomes P-glycoprotein mediated MDR and improves anti-cancer efficiency. Cancer Lett. 2023, 563: 216181.

4) Lei Zhang, Biwei Ye, Yunfeng Lin, Yi-Dong Li, Jing-Quan Wang, Zhuo Chen, Feng-Feng Ping*; Zhe-Sheng Chen* ; Ribociclib inhibits P-gp-mediated multidrug resistance in human epidermoid carci noma cells. Front. Pharmaco, 2022,13:867128.

5) Lei Zhang, Zhuo Chen*. Research progress of structure based antitumor drug design. Chinese J. Struc. Chem. 2020, 39:2085-2090.

6) Lei Zhang, Zhen Li, Xianren Ye*, Zhuo Chen*, Zhe-Sheng Chen*. Mechanisms of thrombosis and research progress on targeted antithrombotic drugs. Drug Discov. Today. 2021 10, 26.

7) Chen He, Lei Zhang*, Wenzhen Liu, Yunmei Huang, Ping Hu, Tao Dai, Jianhua Xu*, Zhuo Chen*. “Albumin-based nanoparticles combined with photodynamic therapy enhance the antitumor activity of curcumin derivative C086.” Dyes Pigm., 2021, 189: 109258.

8) Jihao Liu, Zhuo Chen*, Mingdong Huang, Shuzhi Tang, Qianchao Wang, Ping Hu, Pranav Gupta, Charles R Ashby Jr, Zhe-Sheng Chen*, Lei Zhang*. “Plasminogen activator inhibitor (PAI) trap3, an exocellular peptide inhibitor of PAI-1, attenuates the rearrangement of F-actin and migration of cancer cells.” Exp. Cell Res. 2020, 391, 111987. (IF=3.905)

9) Yunfeng Lin, Zhuo Chen*, Chaohua Hu, Zhe-Sheng Chen, Lei Zhang*, “Recent progress in anti-tumor functions of the intracellular antibodies”, Drug Discov. Today, 2020, 25(6), 1109-1120. (IF=7.851)

10) Lei Zhang, Yidong Li, Qianchao Wang, Zhuo Chen*, Xiaoyun Li, Zhuoxun Wu, Chaohua Hu, Dan Liao, Wei Zhang, Zhe-Sheng Chen*, “The PI3K subunits, P110α and P110β are potential targets for overcoming P-gp and BCRP-mediated MDR in cancer”, Mol. Cancer, 2020, 19:10. (IF=27.401)

11) Lei Zhang, Chaohua Hu, Wendi Yang, Xiaolin Liu, and Yunkun Wu*, “Chemical synthesis, versatile structures and functions of tailorable adjuvants for optimizing oral vaccination”, ACS Appl. Mater. Interfaces, 2016, 8 (51), 34933-34950. (IF=9.229)

12) Lei Zhang, Zhanzhuang Zeng, Chaohua Hu, Susan L. Bellis, Wendi Yang, Yintao Su, Xinyan Zhang, Yunkun Wu*, “Controlled and targeted release of antigens by intelligent shell for improving applicability of oral vaccines”, Biomaterials, 2016, 77 (14), 307-319. (IF=12.479)

13) 胡朝华 , 张蕾* , 张又弛 , 万顺刚, “非均相高效催化剂在餐厨废油转化生物柴油中的应用”,《环境工程》. 2016,9, 105-109.

14) Lei Zhang*, Yiwen Fan, Yunkun Wu*, “Inositol based non-viral vectors for transgene expression in human cervical carcinoma and hepatoma cell lines”, Biomaterials, 2014, 35(6), 2039-2050. (IF=12.479)

15) Lei Zhang#, Wendi Yang#, Chaohua Hu, Qianchao Wang, Yunkun Wu*, “Properties and applications of nanoparticle/microparticle conveyors with adjuvant characteristics suitable for oral vaccination”, Int. J. Nanomed., 2018, 13, 2973-2987. (IF=6.400)

16) Lei Zhang*, Susan L Bellis, Yiwen Fan, Yunkun Wu*, “Using inositol as a biocompatible ligand for efficient transgene expression”, Int. J. Nanomed., 2015, 10(1), 2871-2884. (IF=6.400)

17) Lei Zhang*, Chaohua Hu, Yiwen Fan, Yunkun Wu*, “Binary gene vectors based on hyperbranched poly(L-lactide-co-polyglycerol) and polyethylenimine for prolonged transgene expression via co-assembly with DNA into fiber core-shell triplexes”, J. Mater. Chem. B, 2013, 1(45), 6271-6282. (IF=6.331)

18) Lei Zhang, Chao-Hua Hu, Si-Xue Cheng*, Ren-Xi Zhuo*, “PEI grafted hyperbranched polymers with polyglycerol as a core for gene delivery”, Colloids Surf. B, Biointerfaces, 2010, 76(2), 427-433. (IF=5.268)

19) Lei Zhang, Chao-Hua Hu, Si-Xue Cheng*, Ren-Xi Zhuo*, “Hyperbranched amphiphilic polymer with folate mediated targeting property”, Colloids Surf. B Biointerface, 2010, 79(2), 427-433. (IF=5.268)

20) Chao-Hua Hu, Lei Zhang, De-Qun Wu, Si-Xue Cheng, Xian-Zheng Zhang*, Ren-Xi Zhuo*, “Heparin-modified PEI encapsulated in thermosensitive hydrogels for efficient gene delivery and expression”, J. Mater. Chem., 2009, 19(20), 3189-3197. (IF=6.6)

21) Lei Zhang#, Chaohua Hu#, Weixing Ye, Duanwei Zhu, Ziniu Yu, Ren-XiZhuo, Ming Sun*, “The copper-resistant bacterium ACU isolated from the rhizosphere of Eichhornia crassipes (Mart.) increased the endurance of Potamogeton crispus L. to copper toxicity”, J. Appl. Microbiol., 2008, 105(4), 1034-1043. (IF=3.772)

22) Lei Zhang, Lifang Ruan, Chaohua Hu, Huaiguang Wu, Shouwen Chen, Ziniu Yu, Ming Sun*, “Fusion of the genes for AHL-lactonase and S-layer protein in Bacillus thuringiensis increases its ability to inhibit soft rot caused by Erwinia carotovora”, Appl. Microbiol. Biotechnol., 2007, 74(3), 667-675. (IF=4.813)

23) 孙明，张蕾，朱晨光，喻子牛。“细菌表面S-层的结构及其功能和应用前景”。微生物学报，2002, 42(4), 506-509

24) 胡朝华，张蕾，喻子牛。“植物螯合肽的生物合成与解毒机制及在重金属修复中的应用前景”。华中农业大学学报，2006, 25(5)

25) 张蕾，程巳雪，张先正，卓仁禧。“以聚丙三醇为核枝接PEI的高支化聚合物实现高

效转基因表达“。 全国博士后微纳米粉体与材料应用技术交流会，中国北京，2009.3.1-20

09.3.2 (会议论文摘要)

#为共同一作，*为通讯作者

论著章节:

1) Qingbin Cui*, Pranav Gupta, Lei Zhang, Zhe-Sheng Chen, “BCR-ABL Inhibitors as Sensitizing Agents for Cancer Chemotherapy”, In book, Protein Kinase Inhibitors as Sensitizing Agents for Chemotherapy, Zhe-Sheng Chen, Dong-Hua Yang, ELSSEVIER, pp 29-43, 2019

2) Ming Sun, Lei Zhang, Ziniu Yu, “Molecular biology of Bacillus thuringiensis”, In book, Advances in Microbial Control of Insect Pest, Upadhyay R K, Kluwer Academic/Plenum Publishers, pp 15-40, 2002

授权专利：

1)  “一种pH敏感的双壳层载药缓释体系及其制备方法和用途”, 中国发明专利, 专利号：ZL201510063237.9

2) “一种大肠杆菌分泌表达外源蛋白的质粒载体及其构建方法”, 中国发明专利, 专利号：ZL201410070632.5

3) “一种核壳型温度和pH双敏性金属离子印迹纳米凝胶及其制备方法”, 中国发明专利, 专利号：ZL201310373052.9